FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8187b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8187b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6358665
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT139460.21932276664991787TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71380.11225626762850378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22550.034.92534611
ACACGTC23250.033.874113
AGTCACG22400.033.48938828
CACGAGT22550.033.36443731
CACACGT23850.033.20639812
ACGAGTG23000.032.6165232
CACGTCT24000.032.4488814
ACGTCTG24350.032.07281515
TCACGAG23700.031.83831430
GTCACGA23800.031.70429229
CGAGTGG24050.031.10104433
AGCACAC26400.030.41513810
GATCGGA24800.029.9972251
CAGTCAC26150.029.44402927
CGTCTGA27100.029.3862116
GAGCACA27750.029.2528179
TATCTCG24050.028.9052241
GAACTCC27000.028.59856421
TCCAGTC27250.027.69035125
GAGTGGA27700.027.24115234
Produced by FastQC (version 0.11.2)