FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8187a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8187a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5475514
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG9050.027.71271933
GCACACG10950.024.9123411
GATCGGA11350.023.847081
CGTCTGA12100.022.90826216
ACGTCTG12250.022.44816815
ACACGTC12200.022.35984813
CACACGT12500.021.82321212
CGTACGT11600.021.81035434
CACGTCT12850.021.57120514
GTCACCG13450.019.7918329
CAGTCAC13950.019.39785827
TCCAGTC14950.019.13026625
ACCGTAC13600.018.44118532
CCAGTCA14800.018.43227226
AGTCACC15000.017.89334128
AGCACAC15650.017.85222810
GTACGTA14600.017.47945835
GAGCACA16100.017.3534099
GAACTCC16650.016.78002221
TCACCGT15750.016.62238130
Produced by FastQC (version 0.11.2)