FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8038i-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8038i-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157655
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT84610.13740620414752044TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG20150.033.5177328
GCACACG20700.033.37056411
ACACGTC20900.032.94623613
GTCACGT20800.032.15300429
GATCGGA21150.031.9436021
CGTTTCG20800.031.5186633
ACGTCTG22150.031.08671615
CACACGT22000.030.99868412
GTTTCGG21300.030.77878634
CACGTCT22600.030.3703914
CGTCTGA23150.030.02896116
GAGCACA25000.027.9828139
AGCACAC25000.027.89481510
CAGTCAC24850.027.62100627
CCAGTCA25650.026.58821326
TCCAGTC26050.026.51775425
GAACTCC26750.025.90544321
AGAGCAC27950.025.1080578
GTCTGAA28150.024.7733717
TCGGAAG29450.024.5817953
Produced by FastQC (version 0.11.2)