FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8038a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8038a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6603263
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT163140.24705967337663215TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29750.036.01162311
ACGTGGC29100.035.60806332
GTCACGT29950.035.5521329
AGTCACG30000.035.4926128
CACGTGG29550.035.21470631
TCACGTG30450.034.75187330
ACACGTC31250.034.07187713
GATCGGA31350.033.555881
CACACGT32050.033.3586912
CGTGGCC31550.032.91266633
ACGTCTG32400.032.86253415
CGTCTGA33100.032.30048416
CACGTCT32800.032.26056314
AGCACAC33850.031.7147910
GGCCTTA31900.031.58604236
CAGTCAC33800.031.43699527
TATCTCG30000.031.01937141
GAGCACA35250.030.5176079
AGAGCAC35900.030.4552828
CCAGTCA35900.029.9044626
Produced by FastQC (version 0.11.2)