FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah7797k-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah7797k-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786902
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC307550.31424653072034436TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG50550.037.99329829
TCACGGC50650.037.78817430
GCACACG52700.037.1518111
CACGGCT51600.037.0494531
AGTCACG52350.036.8128228
CACACGT53700.036.33762712
GATCGGA52150.035.701041
ACACGTC54700.035.67368713
CGGCTAC53400.035.34778233
CACGTCT55200.035.31088314
ACGTCTG55650.035.06487715
ACGGCTA55000.034.67947832
CATCTCG47150.033.8266639
CGTCTGA57900.033.66425716
CGTATGC54850.033.61047444
GAACTCC58200.033.3005421
AGCACAC59300.033.01670510
CAGTCAC58600.032.96162427
GGCTACA54950.032.7893134
GAGCACA60100.032.5407759
Produced by FastQC (version 0.11.2)