FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah7797j-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah7797j-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6009907
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC411190.6841869599646051TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG70050.041.29776811
CACACGT72450.039.93005412
ACGTCTG72500.039.81148515
ACACGTC72600.039.75665313
GATCGGA68400.039.703341
CACGTCT73000.039.5990814
CGTCTGA73350.039.289516
AGCACAC73900.039.2653410
CAGTCAC73850.039.0234927
AGAGCAC75400.038.5127338
GAGCACA75900.038.2886439
TCCAGTC74950.038.27466225
CACTAGC75550.038.05899431
TATCTCG59400.037.8509739
CCAGTCA75750.037.84139626
GAACTCC76450.037.609721
TCGGAAG77750.037.4967083
CGTATGC65350.037.46845644
TCACTAG77250.037.3923230
GTCACTA76950.037.36625329
Produced by FastQC (version 0.11.2)