FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah6978b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah6978b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6518612
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC372880.5720236148431599TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56150.040.86322811
AGTCACG56650.040.1928328
ACACGTC57550.039.7547913
ACGTCTG57700.039.46111715
GTCACGA57300.039.42974529
CACGTCT57950.039.3285414
CGATCAG57200.039.3069833
CACACGT58550.039.26366812
AGCACAC59300.038.8038810
CGTCTGA58750.038.64351716
CACGATC58150.038.58915331
GAACTCC59450.038.151521
GATCGGA56700.038.0776981
GATCTCG49250.037.9679839
GAGCACA60550.037.930739
CGTATGC59250.037.7607644
CAGTCAC60900.037.35178827
CCAGTCA60900.037.24341626
TCCAGTC61800.036.7363525
AGAGCAC63250.036.4158948
Produced by FastQC (version 0.11.2)