FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah6978a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah6978a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6462561
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC302250.46769384459195046TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61700.039.1483211
ACACGTC62000.039.0307613
CACACGT62300.038.9840612
ACGTCTG62250.038.7679915
CGTCTGA62350.038.7058116
GTCACAC60750.038.49429
CAGTCAC62450.038.11543327
CACGTCT63800.037.895114
GATCGGA59400.037.7941671
CGTATGC49550.037.64941444
TCCAGTC63600.037.1841125
AGCACAC65850.036.61458210
CCAGTCA64900.036.54097426
GAGCACA65950.036.4589929
AGTCACA65700.036.06254228
GAACTCC66200.035.75610721
TCACACT66450.035.48998630
AGAGCAC68650.035.1211978
GTCTGAA68900.035.02623417
ACACTTG67600.034.62535532
Produced by FastQC (version 0.11.2)