FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah6408k-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah6408k-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7279117
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC217870.2993082814852406TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39800.038.4704311
CTTAGGC39300.038.2899933
ACACGTC41950.036.65658613
CACACGT42000.036.56006612
ACGTCTG42550.036.449915
ACTTAGG41950.036.13341532
CACGTCT43100.035.78059414
AGCACAC43250.035.5542810
CAGTCAC44000.034.54897727
CATCTCG33650.034.51856239
GAACTCC44200.034.4917221
CGTCTGA44850.034.3847116
TTAGGCA43250.034.1314834
GAGCACA45600.033.722229
TCCAGTC46050.033.20182825
AGTCACT46450.032.96349728
CGTATGC41850.032.90703244
GATCGGA43650.032.8212971
TAGGCAT42600.032.48327335
CCAGTCA47300.032.3709126
Produced by FastQC (version 0.11.2)