FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah6408j-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah6408j-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522145
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC231650.41949278767580356TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40350.039.9086111
CACACGT43000.037.7056112
ACGTCTG43000.037.2451615
GATCGGA40150.036.94691
ACACGTC43650.036.94253513
CACGTCT43600.036.88398714
CACGATC30950.036.25070636
AGCACAC44800.036.04268610
GAGCACA45300.035.8876769
GAACTCC44250.035.74527721
CGTCTGA44850.035.7091716
GATCTCG31450.035.53480539
CGATCTC31500.035.26888738
CGTATGC43200.035.23978444
CAGTCAC44900.035.22812327
TCCAGTC44650.035.2279725
CATCACG43700.035.03763233
GTCACAT45800.034.34373529
CCAGTCA46250.033.72387726
AGAGCAC48450.033.6906368
Produced by FastQC (version 0.11.2)