FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5419285
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10350.016.57593511
CACACGT10900.015.537744512
ACGTCTG11850.015.22016215
ACACGTC11850.014.66332713
GAACTCC12350.014.0700621
CGTCTGA12500.013.90083416
TATCTCG11800.013.802381541
CAGTCAC12450.013.78126527
CTCGTAT12350.013.3643144
AGCACAC13950.012.92895510
CACGTCT13700.012.68324314
AGTCACA13900.012.50201828
TCCAGTC13150.012.37797925
CCAGTCA14150.011.81454526
GTCACAT13950.011.66910129
ATCTCGT14400.011.46270442
GAGCACA16650.010.8323689
GATCGGA14500.010.6222891
TCTCGTA16000.010.45398743
GTCTGAA16300.010.39027117
Produced by FastQC (version 0.11.2)