FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5558752
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC7500.011.14519929
GCACACG8450.010.15113911
ACACGTC8750.09.5517413
CACACGT9150.09.37454912
GAACTCC8450.09.11057121
TATCTCG8550.08.75250341
CAGTCAC10700.08.63406727
ACGTCTG9550.08.52159515
CACGTCT9450.08.37871814
TCCAGTC10900.08.27339625
CGTCTGA10150.08.23455316
AGGGGGG5454.8301445E-87.67126851
AGCACAC11250.07.42919710
CTCGTAT12650.07.306541444
CACACTG10900.07.266340331
TCTCGTA13300.07.115557743
CCAGTCA12450.07.06683426
GAGCACA12650.06.78098349
AGTCACA12900.06.650107428
ATCTCGT12800.06.534034342
Produced by FastQC (version 0.11.2)