FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4563808
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70210.15384082765970872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT6050.018.91001931
ACGAGTG5800.018.58874732
AGTCACG6100.017.6687428
GTCACGA6500.017.2591729
TCACGAG6850.017.02198630
CGAGTGG6650.016.54524833
AGGGGGG4750.015.2844581
CAGTCAC9350.013.40933627
GAACTCC9850.013.39756121
TATCTCG8450.013.28574741
GCACACG9200.013.14878511
ACGTCTG9150.012.98026115
ACACGTC9800.012.56818913
TCCAGTC10350.012.53824225
CGTCTGA9500.012.50204116
CACACGT9500.012.50204112
CCAGTCA10850.011.55525826
ATCTCGT10450.011.3738442
GAGTGGA11300.010.90653734
CACGTCT11150.010.45470414
Produced by FastQC (version 0.11.2)