FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6701620
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG12300.011.8056671
AGGGGGG4900.011.6742491
CCCCAGG6201.8189894E-128.871561
CCCTCTA11350.08.5292051
CCCACGT6901.8189894E-128.2904091
CCCATAG9150.08.1754091
CCCATGC8100.08.1486921
CCCACTA11650.08.1207141
CCCCCGA2450.0048303868.0821721
CCCATGA11000.08.0005351
GCACACG20350.07.89430544
CCCACAT11500.07.84400221
CCCTAAA15300.07.7652251
CCCCATG5505.689435E-87.6005081
CCCCTAT8600.07.41910031
CGAGCCT3901.0874125E-47.33694539
CCCTTAG8701.8189894E-127.3338231
CCCTAAG5751.2430064E-77.2700511
CACGACG5751.2430064E-77.2700511
CCCCGTA3654.089484E-47.233361
Produced by FastQC (version 0.11.2)