FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777803
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61400.1285109494887085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG10550.015.6430231
AGGGGGG5400.011.409751
CCCTAAA11150.010.8542471
CCCAGAT12800.09.4550671
CCGCGCT2353.2035672E-49.35911713
CCCGCCG2608.537045E-59.3096041
CCCGCGC2403.955748E-49.16413512
CCGCATG7750.09.084123
CAGGAGG7800.09.02409326
ACCCGCG1950.0069027229.02314911
CCCTTAA8400.08.906591
CCCGCAT9050.08.7517132
GCACCCG3553.640691E-68.67384219
CCCTTGT9500.08.5701811
GTGCGGG3851.1763968E-68.56906110
CGCATGG8100.08.4199844
GGCCGGG3452.3751289E-58.2899956
CCCCGCA9600.08.2516941
TCACAGG9250.08.08476123
CCCTGGA4153.4165678E-67.953441
Produced by FastQC (version 0.11.2)