FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6009879
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101660.1691548199223312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG14000.014.6157371
CGTCCCG1903.695029E-511.58491835
TGCCCGG3301.3544068E-69.3306111
CCCCCGA3106.150951E-69.2267231
CCCATCG7100.08.9868081
CGGGGGG3706.6046414E-78.9198051
CCCTTAA12850.08.9036071
AGTCACG9000.08.79816428
CCGACTG4602.2591848E-88.60969431
CCCGGAC3351.6477667E-58.53482213
CCGACCG2608.768158E-48.460834
CTCGTAT11900.08.32167744
CACGTGG8300.08.2178231
ACGTGGC8250.08.00232632
CCCCTAG3054.8130128E-47.93521451
CCCATGA10950.07.8363951
CCCCTGT4501.2865894E-67.82299951
CCCCCTA3105.729618E-47.8072271
GTCACGT10000.07.69903429
CCCTTGA15000.07.6274241
Produced by FastQC (version 0.11.2)