FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6888153
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG4800.012.3770451
ACCCGCG4500.011.24156811
CCCGCGC5651.8189894E-129.34274212
CCGCGCT5253.092282E-119.21668913
CCGCCGT5551.0913936E-119.1170352
CCCTAAA15400.08.7157261
CCTATCG7650.08.6288771
CCCATCG6056.548362E-118.3650191
CCCATAA16750.08.2759941
CCCTGAG5651.10812834E-87.7888971
CAGGGGG3156.823137E-47.680852423
CCGCCCG3807.9357895E-57.5260293
CCCAGGG5851.6781087E-77.1464791
CGCGCTT7101.9663275E-97.12493714
CCCTCGA6501.9441359E-87.10886671
CCCAAGG10900.07.0653871
CGCTGCG5301.972452E-67.054798611
CCCCCTA3450.00178705527.01565171
CCCACGA7253.0158844E-96.98046351
CCCCTTA6053.01623E-76.9102331
Produced by FastQC (version 0.11.2)