FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8046940
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG5950.012.5735111
ACCCGCG4900.012.56843411
CCGCGCT5800.010.61815913
CCCGGAC4601.8189894E-1210.51923313
CCCGCGC6250.09.50173712
TGCCCGG5152.0918378E-108.96873711
CCGCCGT7100.08.67622
CAGGGGG4153.7896643E-78.4801723
CGCTACG5601.0786607E-98.25254734
GCGCCCC3851.0829415E-57.99804457
GCCCGGA6158.1490725E-107.86804412
CCCCGGT5353.4853656E-87.81437871
GCACGTC5953.5524863E-97.76295222
CCCCGCA8151.8189894E-127.556622521
CCCCTAA8201.8189894E-127.513441
CCCATAA21450.07.48842051
CCCATAG11600.07.20809031
GCACACG17100.07.206857744
CCCTGAA21100.07.19550471
CCTAATA26700.07.16972731
Produced by FastQC (version 0.11.2)