FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6873374
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG3900.011.84281111
CCCGGAC3552.6011548E-1011.15178113
CCCGCCG4602.1827873E-1110.0448821
CCCCTCA6100.09.7390661
GCGCCCC3251.100203E-69.4742497
GCCCGGA4204.796675E-99.42591112
TGCCCGG4703.5288394E-109.3590611
CCCGCGC5051.4188117E-109.14593312
CCGCGCT5303.8198777E-119.129498513
CGATCAG5955.456968E-128.87648233
CCGGACA4805.0840754E-98.70587514
CCGCCGT6055.820766E-107.9998382
CCCTAGT5852.5374902E-97.8985391
CCCCTGT5051.0967233E-77.8426811
CAGGGGG3654.822433E-57.833690623
GCCCGGT4052.1149632E-57.60627832
CCCCCGT2650.009748827.4727431
CCCCGTA2950.00303760787.4586691
CCCCCTG2950.00303760787.4586691
CCCTGGC4459.199868E-67.4167661
Produced by FastQC (version 0.11.2)