FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7127755
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG4750.011.1179461
ACCCGCG3807.2759576E-1110.9970411
CCGCGCT4652.7284841E-119.9328113
CCGCCGT5354.7293724E-119.0466422
CCCGCGC4754.2127795E-98.79763212
GCGCCCC3452.3838402E-58.2877417
CCCTTAA12850.07.87701371
CACTCGG4501.2849323E-67.8237391
GTCACAC8850.07.45635229
CGGTCGG3400.00152773917.12042643
CGCGCTT6552.2708264E-87.05153714
CCCTAAA17300.06.8683841
CGCCCCT5801.1113152E-66.8258458
CGTATGC8552.9467628E-106.691872644
CGCTTGG9355.0931703E-116.58660116
CCCCCCA5351.6943583E-56.58071571
CCCTGTC5757.0063197E-66.505611
CGCGGTG4404.810813E-46.49870727
CCCATCG6102.528801E-66.4930631
CGCCGAG4801.9810979E-46.41503058
Produced by FastQC (version 0.11.2)