FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876376
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC5450.012.9205433
CGTATGC6250.010.91555644
ACTTAGG7150.010.77063132
CATCTCG6300.010.13218239
GCCCCGC5800.09.4802813
CAGTCAC9100.09.18480227
TCCAGTC8900.09.14383125
ACCCCGC7950.09.12999920
CGCCCCG5853.6379788E-129.02328112
GGGTCCG7400.08.91673857
CGGTCGG2250.00224388348.79769914
AGGTGTG9250.08.79769912
TCGTATG7800.08.74733343
CGGGGTC7900.08.6329315
TGGGGGG9300.08.5178171
ACCCGCG3106.1830804E-58.51390211
CCCCGCA8550.08.48929721
CCCCTCA5559.058567E-108.32597351
GGTCCGC7800.08.1774878
CCCCCTA2450.00482450438.0832331
Produced by FastQC (version 0.11.2)