FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_gg13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_gg13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904419
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG4400.012.502041
ACCCGCG4250.011.90288511
CCCGCGC4409.094947E-1210.49735712
CCGCGCT4551.8189894E-1110.15129113
CCCGGGT2059.564018E-49.6601131
CGGTCAG3452.4102155E-79.5628915
GCCCGGA3553.6490383E-79.29343512
GGTCAGG4153.949208E-89.00985516
CCGCCGT6005.456968E-128.7997962
CCCGGAC3758.055267E-78.79778513
CCCCTAG3301.3555575E-58.6680811
CTCGGTC4409.211726E-77.997986313
CCGGACA4702.932611E-77.95537614
TGCCCGG4501.2933997E-67.820253411
GCGCCCC3105.75064E-47.8045527
TTCGCGC4905.693546E-77.63118426
CCCACTA11300.07.594161
GCGTGCC4254.0168437E-57.24664645
CCCTTAA12250.07.18484641
ACTCGCC5251.6842114E-67.12587934
Produced by FastQC (version 0.11.2)