FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5129299
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT585731.1419299206382783TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70730.13789408650187873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG82850.040.5959628
TCACGTG83000.040.58271830
GCACACG84450.040.55220411
CACGTGA83450.040.34026731
CACACGT85700.040.03771212
ACGTCTG85350.039.9707415
GTCACGT84400.039.82629429
CGTCTGA85600.039.7769216
ACACGTC86100.039.7750713
GATCGGA83600.039.556121
CGATCTC69350.039.47977440
GATCTCG69600.039.46445541
GAACTCC87250.039.22636821
CACGTCT87050.039.18939214
GAGCACA87550.039.141449
CTCGTAT79200.039.09402544
AGCACAC88050.038.8941910
CAGTCAC87200.038.72141627
ACGTGAA87650.038.43647832
TCCAGTC88150.038.4016825
Produced by FastQC (version 0.11.2)