FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670207
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3068825.412183364734304TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTAT142170.2507315870478803TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACCTCTCGTAT114480.2018973910476284TruSeq Adapter, Index 18 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79680.14052397028891536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTAT71100.12539224758461198TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA428200.043.430011
GCACACG432750.043.37407311
GAGCACA435000.043.114719
ACACGTC435550.043.09523813
AGCACAC435100.043.0943110
CACACGT435650.043.05505412
AGAGCAC439100.042.922528
CGTCTGA437000.042.8020116
CACGTCT439150.042.6864914
ACGTCTG438650.042.68111815
ATCGGAA447000.042.6525342
TCGGAAG446900.042.6420143
ACGTCCG424100.042.52900332
CGTCCGC424600.042.52412833
AGTCACG432600.042.43439528
CACGTCC427050.042.40479331
GTCCGCA417000.042.33828434
CCGCACA398850.042.3274636
GAACTCC435150.042.32332221
GTCACGT432600.042.3193229
Produced by FastQC (version 0.11.2)