FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8535854
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT93150310.912827234392717TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT951881.1151549686768307TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT756240.8859570465942833TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT371720.4354807380726053TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCGCGTAT259270.30374230861961793TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT256010.29992312427086965TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCGCTCGTAT109180.1279075298148258TruSeq Adapter, Index 16 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCCGTCCCGATCTCGTAT107550.12599793764045167TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91080.10670285597668377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1517400.043.3818111
GAGCACA1521700.043.302589
GATCGGA1504250.043.302421
AGCACAC1521500.043.27791610
AGAGCAC1527650.043.265198
ACGTCTG1518400.043.26078815
CACGTCT1521050.043.2328814
CGTCTGA1516950.043.18614616
TCGGAAG1540600.043.138943
ACACGTC1527850.043.09516513
ATCGGAA1543400.043.0939672
CACACGT1528300.043.0767212
CTCGTAT1282900.043.06298444
CGGAAGA1540900.043.0334634
GTCTGAA1520200.042.9604717
CGTCCCG1464650.042.92767335
GAACTCC1508500.042.9209321
TCTGAAC1522100.042.89383318
GTCCCGA1339000.042.86925536
TCACCCG1463200.042.83955430
Produced by FastQC (version 0.11.2)