FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602513
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT123530.16248574648935163TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24500.032.58747511
ACGTCTG26700.030.15028615
CACGTCT27400.029.53979114
ACACGTC27550.029.53862613
CACACGT27800.029.35210612
GATCGGA26600.028.9524361
CGTCTGA28250.028.88550216
CAGTCAC28800.027.1122827
AGCACAC29550.027.0928110
GAGCACA30850.026.0937259
TCCAGTC29850.025.86332525
CACATGT29950.025.56472631
AGTCACA31100.024.82445728
CCAGTCA31250.024.77535826
AGAGCAC32800.024.6094768
GAACTCC32100.024.39355721
GTCACAT31300.024.1759929
TCACATG31700.024.15215130
GTCTGAA35100.023.43629517
TCGGAAG35250.023.0276073
Produced by FastQC (version 0.11.2)