FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7976118
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT390780.48993758617914124TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG59150.038.56057411
CACACGT60250.037.8565612
ACACGTC60700.037.46720513
ACGTCTG62400.036.44669315
CGTCTGA62900.036.29684416
CACGTCT62850.036.1155214
AGTTCCG61950.035.8438334
AGCACAC64550.035.47104310
GATCGGA63000.035.3445781
TATCTCG55900.035.12164341
CCGTATC58350.035.11894638
GAGCACA65600.035.0374039
CAGTCAC64750.035.02508527
GAACTCC65250.034.6529521
TCCAGTC65300.034.59446725
GTTCCGT63650.034.5426935
CTCGTAT58850.034.1774644
AGTCACA66550.034.01143328
CCAGTCA66750.034.0081726
AGAGCAC68000.033.8978278
Produced by FastQC (version 0.11.2)