FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10359869
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT639430.6172182293038647TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG92650.038.4574111
ACACGTC94850.037.5190313
CACACGT95050.037.37066712
ACGTCTG95650.037.2517615
CGTCTGA96050.037.119716
CACGTCT96050.036.9815914
GATCGGA92500.036.7742461
AGCACAC97950.036.37668610
CACAGTC96450.036.10930331
GAGCACA99500.035.743879
TCCAGTC97900.035.61066425
CAGTCAC98500.035.48340627
GTCACAG99250.035.3934229
GAACTCC99800.035.1973521
AGTCACA100350.034.96059828
CTCGTAT91650.034.88970644
ATCTCGT88400.034.733242
TCTCGTA90750.034.63330543
CCAGTCA101000.034.49588826
AGAGCAC104250.034.326238
Produced by FastQC (version 0.11.2)