FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5825626
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14250.025.00441411
ACGTCTG15650.022.48690215
CACGTCT15650.022.48651714
ACACGTC15800.021.99462313
CACACGT16450.021.25924112
CGTATGC15550.021.0859144
CGTCTGA17500.020.23540116
AGCACAC17450.020.1669910
CAGTCAC18250.019.7655427
TCCAGTC18200.019.33626425
CTCGTAT15950.019.31789442
GTCACCT17900.019.2930329
AGTCACC18400.019.2461228
GAACTCC18650.018.86986721
GATCGGA17500.018.8614391
GAGCACA18900.018.8526959
TCGTATG17750.018.84647843
ATCTCGT15400.018.72305140
TCTCGTA15750.018.58663741
CCAGTCA19450.018.31974426
Produced by FastQC (version 0.11.2)