FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5183625
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC116690.22511273481395738TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25450.034.3075811
ACACGTC26050.033.60181413
ACGTCTG26700.032.94885315
CACACGT27150.032.32142312
GATCGGA26600.032.2580381
CGTCTGA27300.032.224716
AGCACAC27850.031.58800310
CGTATGC23350.031.3879744
CACGTCT28450.030.76721614
GAGCACA28850.030.7217949
TCCAGTC28100.030.60433625
GAACTCC28600.030.52953721
CAGTCAC28400.030.5136827
CATCTCG20950.030.367739
CCAGTCA28950.029.78173426
AGAGCAC30650.029.0613738
AGTCACC29850.028.81066728
GTCTGAA31800.027.73376317
CCAGATC29950.027.70183233
TCGGAAG32550.027.6403473
Produced by FastQC (version 0.11.2)