FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5035681
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC349930.6949010471473471TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC43500.040.7569629
AGTCACG44450.040.0826428
TCACGCC44450.039.64200230
CACACGT47300.038.36277412
ACGTCTG47750.038.1858715
GCACACG47550.038.1610811
CACGCCA46400.038.02531431
ACACGTC48000.037.89496213
CACGTCT49250.037.111814
CGTATGC47000.036.98847644
CGTCTGA49550.036.84308216
GATCGGA48950.036.4543461
GAACTCC49500.036.3033821
CAGTCAC49050.036.18908327
TATCTCG46850.036.17683439
GAGCACA50700.035.9636549
TCCAGTC50400.035.8743825
AGCACAC51350.035.46558410
CTCGTAT47700.035.20495242
CGCCAAT50250.035.16199533
Produced by FastQC (version 0.11.2)