FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4965258
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC225370.45389383592957305TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81970.1650870911441057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC44900.038.8026329
ACGTCTG45750.038.4605615
GCACACG46200.038.0855611
CACACGT46300.037.90829512
CACACAG45700.037.7455131
ACACGTC46900.037.32953313
CACGTCT47300.037.2928514
CGTCTGA47500.036.85874616
CAGTCAC47450.036.80932227
AGTCACA47350.036.79340728
CGTATGC44000.036.56022644
GATCTCG31150.036.24748639
AGCACAC48450.036.2260910
TCCAGTC48500.036.10131525
GATCGGA46900.035.7934841
GAGCACA49050.035.7388349
CCAGTCA48950.035.54546726
CACAGTG49050.035.35341633
AGAGCAC50600.034.7748228
GAACTCC50150.034.43036721
Produced by FastQC (version 0.11.2)