FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7234374
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC140830.1946678454832443TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26400.032.82417711
ACACGTC27150.032.3224813
ACGTCTG28100.031.30777715
CACGTCT28050.030.9715414
ACTGACC27200.030.90190132
CGTCTGA28200.030.80700916
CACACGT28350.030.6440112
AGCACAC30850.028.73104510
GATCGGA30300.028.5387291
GAGCACA32550.027.1627459
CGTATGC31450.027.0116144
CTGACCA31150.026.98634533
CAGTCAC32100.026.86133827
GAACTCC32550.026.55535721
TCCAGTC32400.026.54344725
CCAGTCA33800.025.44419126
TCGTATG33600.025.0898143
AGTCACT34700.024.97614128
TCTCGTA29150.024.91869441
GTCTGAA35000.024.82147617
Produced by FastQC (version 0.11.2)