FastQCFastQC Report
Tue 15 May 2018
H2L35AFXY_n01_fk01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L35AFXY_n01_fk01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738776
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC165230.287918538726725TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63330.110354542501746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28050.037.00950611
CACACGT29100.035.4473712
ACACGTC29700.034.80531313
CCGATGT28650.034.71753333
CGTCTGA29900.034.49924516
ACGTCTG30150.034.2861315
CACGTCT30900.033.4536514
TATCTCG26050.032.6991739
GATCGGA30800.032.575031
GTCACCG31550.032.4209629
CAGTCAC32000.032.2380427
CACCGAT31950.031.81433931
CGTATGC29400.031.36376844
TCACCGA32600.031.24558330
AGTCACC32700.031.21105428
ACCGATG32450.031.12479632
AGCACAC33350.031.06198510
CGATGTA31750.030.84665934
GAGCACA33800.030.583379
CCAGTCA33750.030.23951526
Produced by FastQC (version 0.11.2)