FastQCFastQC Report
Thu 18 Jan 2024
H2L2LDSXC_l04_n01_CE_QG4445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L2LDSXC_l04_n01_CE_QG4445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8597116
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCTCGTAT968981.127098901538609TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCTCGTTT632840.7361073178493811TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCTCGGTT465150.5410535346969845TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCGCGTAT441440.513474518664166TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCTCGGAT389790.4533962319456897TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCGCGTTT291710.3393114621228793TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCGCGGTT161030.18730699923090488TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCTCGATCGCGGAT125160.14558370504713442TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)