FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9724a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9724a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282504
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC42050.012.76355250-51
ACGTGGC49250.011.28320332-33
CGTATGC50300.011.09493446-47
TATGCCG50100.010.80747148-49
GGCCTTA51400.010.5802336-37
CTCGTAT52400.010.37836844-45
TCACGTG57900.09.88474530-31
AGTCACG59200.09.66772828-29
CCGTCTT58600.09.56410952-53
ATCTCGT58200.09.42570542-43
AAGGGGG37750.09.31151574-75
GTGGCCT64250.09.05556834-35
AGGGGGG27250.08.10572774-75
GCCGTCT40450.07.925842352-53
GTATGCC45000.07.388265648-49
CCAGTCA84600.06.961621326-27
CACGTGG51250.06.579869332-33
TATCTCG47850.06.5511442-43
CCTTATC81550.06.46476638-39
CGTGGCC52550.06.32671234-35