FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9723i-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9723i-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934022
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC190880.27528034955758723TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA59950.035.969219
GATCGGA59200.035.0845031
AGAGCAC67000.032.2555168
TCGGAAG67500.031.8921833
ATCGGAA78150.027.8512762
CGGAAGA79500.027.436924
AGTCACG46000.026.74256528-29
TCACGGC45700.026.71025730-31
ACGGCTA48250.025.495532-33
CGTATGC48600.024.7743844-45
CACACGT50350.024.52655812-13
CGTCTGA51300.024.44261716-17
CACGGCT44000.024.28795830-31
TGCCGTC49200.024.13454848-49
CACGTCT51900.024.0227714-15
CTCGTAT50050.023.72449942-43
GGCTACA50950.023.67830834-35
TATGCCG51550.023.2184446-47
GTCACGG46950.023.1159528-29
GCCGTCT45000.023.00955648-49
Produced by FastQC (version 0.11.2)