FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9722i-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9722i-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7533432
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC141050.18723206103141304TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG132060.17529858900963066TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG58150.029.64912228-29
TGCCGTC59550.027.8753548-49
CTCGTAT62700.026.05828542-43
CGTATGC65750.025.7163444-45
TATGCCG66950.025.11352246-47
TCACGAT70400.024.65866930-31
ATCTCGT62850.024.44690740-41
AGATCTC63300.024.27311338-39
CCGTCTT70800.023.74788550-51
CCAGTCA73000.023.52024526-27
CACGTCT74200.023.07577314-15
CGTCTGA74550.022.99929216-17
CACACGT77250.022.2569912-13
GAGCACA88950.020.9313839
TCAGATC76600.020.43062236-37
AAGGGGG52050.020.35097372-73
AGCACAC85650.020.10196110-11
TGAACTC88600.019.70068720-21
GATCAGA84950.019.68041434-35
CTCCAGT90050.019.4362124-25
Produced by FastQC (version 0.11.2)