FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9722b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9722b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944775
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23850.010.15660944-45
AAGGGGG23600.09.35880572-73
GAGCACA53500.09.3219589
AGTCACA39000.09.31670228-29
CGTCTGA39100.08.92838316-17
AGAGCAC57650.08.8981398
CCAGTCA41650.08.66693426-27
GTCACAC29900.08.57803828-29
CACGTCT41200.08.47329614-15
CACACGT41600.08.39182112-13
TATGCCG26700.08.3608846-47
TCACACT43800.08.24147230-31
AGGGGGG18600.08.17177772-73
GTATGCC22900.08.08902244-45
ACACTTG45750.08.04592432-33
GATCGGA62600.07.894841
CAGTCAC34100.07.86975126-27
CTCCAGT48350.07.809842624-25
TGCCGTC25050.07.77394648-49
CTCGTAT30650.07.593306542-43