FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9722a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9722a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8505354
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31950.07.7302848-49
AGGGGGG21500.07.622246772-73
GCCGTCT29100.06.77357448-49
CGTATGC35750.06.57645644-45
TATGCCG38700.06.44334146-47
CATCTCG30900.06.378996438-39
GTATGCC32550.06.274515644-45
ACTTAGG40550.06.14937832-33
GCATCTC39500.05.77174238-39
AGGCATC46800.05.632612736-37
CTCGTAT42350.05.49547742-43
GCACACG97350.05.49066494-95
AAGGGGG29750.05.4286872-73
CTTAGGC38200.05.40864732-33
TTAGGCA46600.05.40197734-35
ATGCCGT39750.05.257488346-47
CCGTCTT46350.05.17492950-51
AGATCGG185800.05.11437594-95
ATCTCGT44500.05.01649740-41