FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9210a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9210a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6892372
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA302480.4388619766895925TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT202300.29351288641994366TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC84450.035.26417550-51
TATGCCG88850.033.8921648-49
CGTATGC90000.033.45909546-47
CGATCTC75100.033.3934640-41
CCGTCTT94650.031.46380252-53
CTCGTAT88850.031.058944-45
AAGGGGG61250.030.67213674-75
ATCTCGT85150.029.70313842-43
AACGATC90300.028.19320538-39
AGTCACG110350.027.86966128-29
TCACGTG111400.027.64981330-31
TGCTTGA112000.026.16602160-61
ACGTGAA120450.025.7300832-33
CTTCTGC121000.024.69065956-57
GAAACGA118000.024.37246936-37
CCAGTCA127050.024.20644226-27
GTCTTCT125050.023.9478954-55
GTGAAAC127150.023.85129534-35
TCTGCTT128850.022.87325358-59
CTCCAGT140050.021.9765424-25
Produced by FastQC (version 0.11.2)