FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah9120b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah9120b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6399457
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25000.016.5288248-49
CGATCTC22450.014.70373438-39
CACGATC23750.013.4989336-37
CGTATGC30450.013.0244244-45
TATGCCG33450.012.35328246-47
CCGTCTT36900.011.71325750-51
AAGGGGG24400.011.29097272-73
CTCGTAT32300.011.24910842-43
ATCACGA37450.09.89240734-35
GCCGTCT25900.09.81107548-49
AGGGGGG20100.09.80720472-73
CCAGTCA44750.09.71152126-27
AGTCACA43950.09.67215728-29
ATCTCGT35950.09.24822440-41
GTATGCC30000.08.94512544-45
GATCTCG24950.08.56645638-39
TCACATC51150.08.26425230-31
ATGCCGT32100.08.13798446-47
CATCACG32700.07.98866432-33
CGTCTGA55500.07.744911716-17
Produced by FastQC (version 0.11.2)