FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8584b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8584b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456096
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA133970.20750930593349293TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT124860.19339861117306806TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG48800.031.4860132-33
CCGCACA46900.030.88802736-37
TGCCGTC49100.029.9395850-51
GTCCGCA51000.029.75526834-35
TATGCCG50550.029.55058148-49
CGTATGC50550.029.08055546-47
AGTCACG56200.027.63596328-29
TCACGTC58400.026.18823230-31
CTCGTAT56200.025.6921544-45
GCACATC54100.025.28473538-39
CCGTCTT56800.024.87740952-53
ATCTCGT59000.023.22506742-43
AAGGGGG37300.022.85883574-75
CCAGTCA71700.021.69478226-27
ACATCTC65450.021.11769540-41
CTCCAGT80000.019.59237724-25
CGTCTGA86150.018.0834716-17
TGCTTGA81350.017.66163860-61
CGTCCGC47750.017.60609434-35
CACGTCT90050.017.40577514-15
Produced by FastQC (version 0.11.2)