FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8584a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8584a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562142
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA67000.14686083861484364TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA20750.032.38920236-37
CCCGATC20100.027.41093438-39
ACCCGTC27200.026.6294932-33
CGTCCCG22300.026.19763836-37
CCGTCCC28100.026.03012734-35
TGCCGTC29700.024.70779250-51
TCACCCG29950.024.50195130-31
TATGCCG31850.023.18904748-49
CGTATGC31900.023.00380346-47
CGATCTC23600.022.84261140-41
AGAGCAC55800.022.1319
GCCGTCT27150.021.51776752-53
CCCGTCC27650.020.95687734-35
CTCGTAT31050.020.95660244-45
CCGTCTT35350.020.62437852-53
CCAGTCA39650.019.64590526-27
AAGGGGG20750.019.57279274-75
CACCCGT30400.019.52982332-33
GATCGGA62750.019.3113272
AGTCACC39700.019.2622428-29
Produced by FastQC (version 0.11.2)