FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8583b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8583b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102758
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA107640.17637926983177116TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT62110.10177365709077765TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC35200.025.70457850-51
CGTATGC39200.024.59620946-47
TATGCCG41000.023.74806248-49
AAGGGGG37750.022.33490474-75
CTCGTAT43000.021.5941744-45
ATGTCAG72050.021.55618734-35
ACATGTC72200.021.34694332-33
TCACATG72400.021.22245830-31
CCAGTCA74150.020.4334326-27
AGTCACA75600.020.41847428-29
AGGGGGG33900.020.3179576-77
ATCTCGT48050.020.11542342-43
CCGTCTT44300.018.86979152-53
GTCAGAA81550.018.75383436-37
CTCCAGT83500.018.40134224-25
GAATCTC63500.017.50250840-41
CGTCTGA90850.017.04318816-17
CAGAATC86650.016.47153538-39
GCCGTCT32350.016.4437952-53
CACGTCT95300.016.3719614-15
Produced by FastQC (version 0.11.2)