FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8583a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8583a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8146766
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA206110.2529960968561022TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT97030.11910247575541019TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC56300.028.51581650-51
CGTATGC62650.026.00446346-47
CCGTATC66450.025.62498938-39
TATGCCG64600.025.5137648-49
AGTTCCG75450.024.17360934-35
CTCGTAT66300.023.3904144-45
AAGGGGG46200.023.38971374-75
ATCTCGT68200.022.7388442-43
CCGTCTT72150.021.62588552-53
TTCCGTA84650.021.29379336-37
GTATCTC80050.019.96609940-41
GCCGTCT54200.019.93686952-53
AGTCACA95250.019.49772328-29
CCAGTCA98850.018.88356826-27
GTATGCC61500.018.6131348-49
TCACAGT100650.018.52243230-31
AGGGGGG43200.018.14230276-77
TATCTCG60050.017.91532542-43
ACAGTTC104050.017.78023732-33
ATGCCGT65500.017.11386950-51
Produced by FastQC (version 0.11.2)