FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8104b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8104b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926607
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29750.018.28009250-51
CGTATGC35500.015.18543946-47
TATGCCG42050.012.98947748-49
CTCGTAT41050.012.84309444-45
AAGGGGG31900.011.7636774-75
CCGTCTT45550.011.73070152-53
ATCTCGT44150.011.61857542-43
AGGGGGG22800.011.25036776-77
GCCGTCT30000.010.9241552-53
AGTCAAC56750.010.00142534-35
CCAGTCA55000.09.88786826-27
AGTCACA56850.09.81673928-29
ATGCCGT37150.09.26913150-51
GTATGCC37650.09.01988348-49
TCACAGT63550.08.81914130-31
ACAGTCA64200.08.65586932-33
CAATCTC63950.08.28121840-41
TCGTATG41750.08.1909846-47
TGCTTGA68200.08.18304160-61
CTCCAGT70050.07.865198624-25
Produced by FastQC (version 0.11.2)