FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah8104a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah8104a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7689081
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33300.015.33333548-49
TATGCCG36400.013.57067646-47
CGTATGC41000.012.27956844-45
CCGTCTT44950.011.67629150-51
CTCGTAT41400.011.18575642-43
ATCTCGT42600.010.87066440-41
AAGGGGG29050.010.05596972-73
GCCGTCT30750.010.04015948-49
AGGGGGG23800.09.97902272-73
CCAGTCA59350.09.16320226-27
GTATGCC36650.08.94203144-45
AGTCACC59700.08.75149428-29
TGTAATC62350.08.07477636-37
ACGGGGG7053.3651304E-107.74825172-73
ATGCCGT40150.07.571186546-47
TGCTTGA66600.07.34554658-59
ACCTTGT76000.07.31232-33
TAATCTC66750.07.29346138-39
CTCCAGT79000.07.094451424-25
CTTCTGC74150.06.917957354-55
Produced by FastQC (version 0.11.2)