FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7838red1-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7838red1-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8870922
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33300.011.9096550-51
CGTATGC34500.011.56423746-47
GAGCACA55050.011.56139
TATGCCG36600.011.54956648-49
AGGGGGG20300.011.11502974-75
GCCGTCT31000.010.80150350-51
GTCACAC31750.010.62120628-29
TATCTCG33650.010.51545340-41
CTCGTAT41000.010.36802344-45
AGAGCAC64500.010.0147678
GATCGGA67450.010.0055811
GTATGCC33900.09.94753446-47
CACACGT40950.09.74276312-13
CACGTCT42750.09.55474514-15
TCGGAAG72350.09.5236123
CCAGTCA45200.09.29959526-27
AAGGGGG29900.09.29389574-75
CGTCTGA44450.09.189321516-17
ATCTCGT47150.09.06603842-43
CCGTCTT44850.08.94851652-53
Produced by FastQC (version 0.11.2)