FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7838i-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7838i-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958440
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT26700.013.60818734-35
TGCCGTC28400.011.87389650-51
GAGCACA45950.011.5767259
CGTATGC31300.011.38073146-47
CCGTACG22800.011.35302932-33
AGGGGGG19100.011.19206974-75
CTCGTAT31750.010.92020444-45
ACCGTAC34750.010.79747932-33
TATGCCG33800.010.67948148-49
GATCGGA48350.010.6155551
CACGTCT35950.010.50311914-15
AGAGCAC49950.010.4594888
CGTCTGA35850.010.39993416-17
CACACGT36500.010.2147312-13
AGTCACC38300.09.79666928-29
ATCTCGT35750.09.56554342-43
CCAGTCA41000.09.55696926-27
CGTAATC38350.09.35049938-39
TCGGAAG58550.09.25173
AAGGGGG26250.09.2293774-75
Produced by FastQC (version 0.11.2)